新疆石河子市牛源大肠杆菌分离鉴定及对β-内酰胺类药物的耐药性分析
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S852.6

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新疆生产建设兵团重点领域科技攻关计划项目(2019AB029);新疆生产建设兵团重点领域科技攻关计划项目(2021AB012);石河子大学大学生研究训练计划项目(SRP2020068)


Isolation and Identification of Bovine E.coli in Shihezi City of Xinjiang and Analysis on Its Drug Resistance to β-Lactams
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    摘要:

    为调查石河子市牛源大肠杆菌对β-内酰胺类药物的耐药表型和耐药基因携带情况,采集健康牛群粪便,采用常规细菌分离鉴定方法并结合16S rRNA PCR检测方法进行分离鉴定;参考Clermont方法对分离菌株进行系统分子分群,采用PCR方法检测分离菌株的β-内酰胺类耐药基因blaCTX-M、blaOXA、blaSHV和blaTEM携带情况,通过K-B纸片法测定分离菌株耐药表型。结果显示:分离鉴定出100株牛肠道大肠杆菌分属于A、B1、C和F群;分离株对头孢唑林和阿莫西林的耐药率在50%以上,对氨苄西林、头孢氨苄与头孢吡肟的耐药率均在5%以下,所有分离株对头孢吡肟敏感;5株分离菌株检测到blaSHV基因片段,1株检测到blaCTX-M基因片段,所有菌株均未检测到blaOXA和blaTEM基因片段。结果表明,石河子市牛源正常大肠杆菌已少部分携带耐药基因,且对部分β-内酰胺类药物产生了耐药性。结果提示,应加快减抗政策的实施。

    Abstract:

    In order to investigate the drug resistance phenotype of bovine E.coli against β-lactam drugs in Shihezi and the carrying of relevant gene,the feces of healthy cattle were collected for E.coli isolation and identifion by a routine bacterial method and 16S rRNA PCR;the isolated strains were systematically molecular clustered with the Clermont method,and the β-lactam resistance genes blaCTX-M,blaOXA,blaSHV and blaTEM of the strains were detected by PCR,followed by determination of drug resistance phenotype by K-B disk. The results showed that 100 strains of E.colis were isolated and identified,falling into groups A,B1,C and F,respectively;the resistance rates of the isolates to cefazolin and amoxicillin were above 50%,and less than 5% to ampicillin,cefalexin and cefepime,all of which were sensitive to cefepime;blaSHV gene fragment was detected from 5 strains,blaCTX-M from one strain,neither blaOXA nor blaTEM from all strains. In conclusion,drug resistance gene was carried in some of normal bovine E.coli,and produced resistance to certain β-Lactam drugs,calling for rapid implementation of the policies for reduction of antibiotics use.

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许淑芸,何白雪,俞杰,马雪,胡晓彦,邹思颖,李劼,周霞,黄新,钟发刚,吴桐中,韩猛立,张星星.新疆石河子市牛源大肠杆菌分离鉴定及对β-内酰胺类药物的耐药性分析[J].《中国动物检疫》编辑部,2022,39(7):49-54.

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