In order to compare the efficacy of commercial ELISA kits and AGID kits in detection of antibodies against equine infectious anemia virus（EIAV），and to evaluate the sensitivity，specificity and consistency of different ELISA kits，EIAV strong positive serum was diluted in multiple ratio to detect by four ELISA kits and three AGID kits respectively，and to compare the lowest detection limit；in addition，the detection effects of the former was comprehensively evaluated through calculating the coincidence rate，Kappa value，sensitivity，specificity and Youden index，taking 28 clinical horse sera as sample plates and the results by AGID as standards. For sensitivity，it was shown that the maximum dilutions of the four ELISA kits were 1:1 024，1:256，1:32 and 1:128 respectively，while those of the three AGID kits were 1:16. For clinical samples，it was shown that the coincidence rate of the results by all the ELISA kits was above 89%，especially higher in three ELISA kits；the sensitivity，specificity and Youden index of the four ELISA kits were 75.00%~100%，87.50%~100% and 0.75~1.00，respectively. In conclusion，the sensitivity of ELISA kits was generally higher than that of AGID kits，sharing higher coincidence rate with AGID kits，which could be used as one of the reliable means for detecting EIAV. However，the sensitivity and specificity were different to certain extent depending on different ELISA kits that should be reasonably selected and used according to specific conditions. Thus，future development of any scientific and reasonable detection plan for EIAV and selection of an appropriate kit were supported with the data obtained in the paper.