不同ELISA和AGID试剂盒检测马传染性贫血病毒抗体的比较
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S855

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国家标准样品研复制项目(S2021063);新疆维吾尔自治区少数民族特培项目(201523128)


Comparison of Different ELISA and AGID Kits for Detecting Antibodies against Equine Infectious Anemia Virus
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    摘要:

    为比较商品化ELISA试剂盒和AGID试剂盒检测马传染性贫血病毒(EIAV)抗体的效果,并评价不同ELISA试剂盒的敏感性、特异性和一致性,将EIAV强阳性血清进行倍比稀释,分别用4种商品化ELISA试剂盒和3种AGID试剂盒进行检测,比较最低检出限;此外,以28份临床马血清作为样品盘,以AGID结果作为标准,通过计算符合率、Kappa值、敏感性、特异性和约登指数,综合评价4种ELISA试剂盒的检测效果。灵敏性结果显示:4种ELISA试剂盒可检出的最大稀释度分别为1:1 024、1:256、1:32和1:128,而3种AGID试剂盒可检出的最大稀释度均为1:16。临床样本检测结果显示:4种ELISA试剂盒与AGID检测结果的符合率均超过89%,其中有3个品牌的ELISA试剂盒一致性较高;4种ELISA试剂盒敏感性为75.00%~100%,特异性为87.50%~100%,约登指数为0.75~1.00。结果表明,ELISA试剂盒的检测灵敏度普遍高于AGID试剂盒,且与AGID试剂盒的检测结果符合率高,是EIAV检测的可靠手段之一,但不同ELISA试剂盒敏感性和特异性存在一定差异,应根据实际情况合理选择使用。本研究为制定科学合理的EIAV检测方案和试剂盒筛选提供了数据支撑。

    Abstract:

    In order to compare the efficacy of commercial ELISA kits and AGID kits in detection of antibodies against equine infectious anemia virus(EIAV),and to evaluate the sensitivity,specificity and consistency of different ELISA kits,EIAV strong positive serum was diluted in multiple ratio to detect by four ELISA kits and three AGID kits respectively,and to compare the lowest detection limit;in addition,the detection effects of the former was comprehensively evaluated through calculating the coincidence rate,Kappa value,sensitivity,specificity and Youden index,taking 28 clinical horse sera as sample plates and the results by AGID as standards. For sensitivity,it was shown that the maximum dilutions of the four ELISA kits were 1:1 024,1:256,1:32 and 1:128 respectively,while those of the three AGID kits were 1:16. For clinical samples,it was shown that the coincidence rate of the results by all the ELISA kits was above 89%,especially higher in three ELISA kits;the sensitivity,specificity and Youden index of the four ELISA kits were 75.00%~100%,87.50%~100% and 0.75~1.00,respectively. In conclusion,the sensitivity of ELISA kits was generally higher than that of AGID kits,sharing higher coincidence rate with AGID kits,which could be used as one of the reliable means for detecting EIAV. However,the sensitivity and specificity were different to certain extent depending on different ELISA kits that should be reasonably selected and used according to specific conditions. Thus,future development of any scientific and reasonable detection plan for EIAV and selection of an appropriate kit were supported with the data obtained in the paper.

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薛文,秦菊,努尔买买提·阿穆提,马晓艳,苏晓慧,阿依吐拉·肉孜,李雷斌,陈玲玲,古丽曼·木哈买提拜,王文.不同ELISA和AGID试剂盒检测马传染性贫血病毒抗体的比较[J].《中国动物检疫》编辑部,2022,39(7):103-107.

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