为估计新疆和静县巴音布鲁克羊新孢子虫流行率，了解其在羊群中的感染情况，通过横断面研究方法，采用两阶段抽样策略，在和静县下辖的12个乡镇中抽取67个养殖场点，采集1 340份血清样品，使用间接ELISA方法检测新孢子虫抗体；通过问卷调查，分析群间传播相关风险因素。抗体检测结果显示，和静县巴音布鲁克羊新孢子虫感染的群表观流行率为32.84%（95%CI：21.85%~45.40%），真实流行率为 36.05%（95%CI：24.93%~49.93%）；12个乡镇中，有8个检出阳性场群。对问卷调查结果，进行卡方检验和Logistic回归分析发现，“饲养方式为舍饲”“建厂时间在6年以上”和“圈舍没有消毒”等因素具有统计学意义（P＜0.05），为养殖场感染新孢子虫的主要风险因素。结果表明：新孢子虫在和静县巴音布鲁克羊群中流行普遍，流行范围较广；长时间舍内饲养、建厂时间久、卫生消毒管理差是导致新孢子虫流行的主要风险因素。因此，提高养殖场户饲养管理水平，定期对圈舍进行消毒并保证一定的空圈时间，可降低新孢子虫的感染风险。
In order to estimate the prevalence of neosporosis in Bayanbulak sheep in Hejing County，Xinjiang，a total of 1 340 serum samples were collected from 67 breeding premises in 12 townships（towns）within the county using a two-stage sampling strategy and through cross-sectional study to detect the antibodies against Neospora by indirect ELISA；then the risk factors related to inter group transmission were analyzed through a questionnaire investigation. It was shown that，after antibody detection，the apparent prevalence at herd level was 32.84%（95%CI：21.85%-45.40%），and the true prevalence was 36.05%（95%CI：24.93%-49.93%）；positive farms/herds were detected from 8 out of 12 townships（towns）. The data collected through questionnaire was analyzed by the chi-square test and Logistic regression. It was shown that the factors including“house feeding”，“being established 6 years ago”and “no disinfection in sheep pens”were of statistical significance（P＜0.05），which were the major risk factors leading to neosporosis in farms. In conclusion，neosporosis was widely prevalent in Bayanbulak sheep in Hejing County，which was mainly caused by the major risk factors including house feeding for a long time，old establishment and poor management of sanitation and disinfection，thus could be controlled through improving the level of breeding management in the farms/households，regularly disinfecting their pens and leaving a certain period of vacancy.