宁夏某肉牛场牛呼吸道疾病综合征相关病原检测及分型鉴定
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

S852.65

基金项目:

“十四五”国家重点研发计划项目(2021YFD1600203);国家农业产业技术体系四川肉牛创新团队专项(SCCXTD-2020-13);四川省高等学校重点实验室项目(2021PTJS34)


Detection and Typing of Pathogens Related to Bovine Respiratory Disease Complex in a Beef Cattle Farm in Ningxia
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    2022年3月冬春换季期间,宁夏某肉牛场暴发牛呼吸道疾病综合征(bovine respiratory disease complex,BRDC)。为了解造成此次病情的病原,采集12份发病牛的鼻腔深部棉拭子样品,采用PCR和RT-PCR方法,分别对牛副流感病毒3型(BPIV-3)、牛冠状病毒(BCoV)、牛病毒性腹泻病毒(BVDV)、牛传染性鼻气管炎病毒(IBRV)、牛腺病毒3型病毒(BAdV-3)、牛呼吸道合胞体病毒(BRSV)、牛细小病毒(BPV)、多杀性巴氏杆菌(P. multocida)、溶血性曼氏杆菌(M. haemolytica,Mh)和牛支原体(M. bovis)共10种BRDC相关病原进行核酸检测。结果显示:共检出4种病原,其中BAdV-3和Mh的检出率均为100%,BCoV和BVDV检出率均为66.67%,其他病原均未检出;在12份牛鼻拭子样品中,存在BAdV-3+Mh、BAdV-3+Mh+BVDV、BAdV-3+Mh+BCoV和BAdV-3+Mh+BCoV+BVDV共4种不同组合形式的混合感染,混合感染率分别为16.67%、16.67%、25.00%和41.67%。对Mh和BVDV进行分型鉴定发现,该场流行的Mh为荚膜血清型A1型,BVDV为基因1a亚型。结果表明,该牛场发生的BRDC是由BAdV-3、BCoV、BVDV 1a亚型和Mh A1型等病原以不同组合形式混合感染所致。因此,牛场应加强生物安全管理和饲养管理,制定合理免疫程序,做好不同病原混合感染的综合防控。

    Abstract:

    During the period when winter turned to spring in March 2022,an outbreak of bovine respiratory disease complex(BRDC)occurred in a beef cattle farm in Ningxia. In order to detect the pathogens,12 swabs were collected from deep nasal cavity of infected cattle to detect the nucleic acids of 10 pathogens related to BRDC,including bovine parainfluenza virus type 3(BPIV-3),bovine coronavirus(BCoV),bovine viral diarrhea virus(BVDV),infectious bovine rhinotracheitis virus(IBRV),bovine adenovirus virus type 3(BAdV-3),bovine respiratory syncytial virus(BRSV),bovine parvovirus(BPV),Pasteurella multocida(P. multocida),Mannheimia haemolytica(M. haemolytica,Mh)and Mycoplasma bovis(M. bovis).The results showed that four pathogens were detected,specifically,both BAdV-3 and Mh were 100%,both BCoV and BVDV were 66.67%,and no other pathogens were found;for the 12 samples,mixed infection was observed,including BAdV-3+Mh,BAdV-3+Mh+BVDV,BAdV-3+Mh+BCoV and BAdV-3+Mh+BCoV+BVDV,and the mixed infection rates were 16.67%,16.67%,25.00% and 41.67% respectively. It was found that,by typing and identification for Mh and BVDV,the prevalent Mh strain fell into capsular serotype A1,and BVDV belonged to gene 1a subtype. In conclusion,the BRDC occurred in the farm was caused by mixed infection with BAdV-3,BCoV,BVDV 1a subtype and Mh A1 serotype in different combinations. Therefore,the management of biological safety and breeding should be strengthened,reasonable immune procedures should be formulated,different pathogens mixed infection should be comprehensive controlled in beef farms

    参考文献
    相似文献
    引证文献
引用本文

常益铭,岳华,汤承.宁夏某肉牛场牛呼吸道疾病综合征相关病原检测及分型鉴定[J].《中国动物检疫》编辑部,2022,39(11):29-34.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2022-11-03
  • 出版日期:
您是第位访问者
《中国动物检疫》编辑部 ® 2022 版权所有
地址:山东省青岛市南京路369号《中国动物检疫》编辑部 邮编:266032
电话:0532-85623545 0532-85622559 0532-85642906 传真:0532-85621826 鲁ICP备08016398号-3
技术支持:北京勤云科技发展有限公司