为深入了解云南省牛冠状病毒（bovine coranarirus，BCoV）在不同地区、不同年龄牛群中的流行情况，2021—2022年采集大理、陆良、石林、寻甸等4个地区5家牛场不同年龄牛的粪便样品357份（腹泻样品61份、非腹泻样品296份）进行BCoV RT-PCR检测，并对BCoV阳性样品进行N基因测序分析。结果显示：在357份粪便样品中，检出BCoV阳性51份，总样品检出率为14.29%，5家牛场均有阳性检出，场阳性率为100%；1周龄、1周龄—1月龄、1~6月龄、1岁以上牛粪便样品的BCoV检出率分别为10.00%、19.23%、10.81%、17.31%；腹泻样品BCoV检出率为36.07%，非腹泻样品为9.78%。通过扩增得到4条完整的BCoV N基因序列；经同源性与遗传进化分析，4条N基因序列高度保守，相互间核苷酸同源性为99.0%~99.8%，与国内参考毒株处于同一小分支，亲缘关系近，核苷酸同源性为97.7%~99.9%，与美国4-17-03、7-16-23、4-17-25和日本GF2020等毒株处于同一个大分支且遗传距离较近；与美国原始毒株Mebus处于不同的大分支，亲缘关系较远，核苷酸同源性为98.1%~98.2%。结果表明：云南省牛群中可能广泛存在BCoV流行，1周龄—1月龄犊牛感染发病风险最高，调运或引种导致BCoV在国内传播的可能性较大。因此，需加强BCoV流行的监测与控制，严格进行检疫，加快疫苗研发和应用。
In order to investigate the prevalence of bovine coronavirus（BCoV）in different regions and cattle population at different ages in Yunnan Province，357 fecal samples（61 diarrhea and 296 non-diarrhea samples）were collected from the cattle at different ages in 5 farms in Dali，Luliang，Shilin and Xundian from 2021 to 2022 to detect BCoV by RT-PCR，followed by analysis on the N gene sequence for BCoV positive samples. The results showed that，for the 357 samples，51 positive samples were detected，with a total detection rate of 14.29%，and they were from all the 5 farms，with a farm positive rate of 100%；the detection rates of the samples from the cattle at 1 week old，1 week to 1 month old，1 to 6 months old and over 1 year old were 10.00%，19.23%，10.81% and 17.31% respectively；those of diarrhea and non-diarrhea samples were 36.07% and 9.78%，respectively. Four complete BCoV N gene sequences were obtained by amplification，which，by analysis on homology and genetic evolution，were highly conserved，with a nucleotide homology from 99.0% to 99.8%，locating at the same sub-branch with the domestic reference strain，with close genetic relationship and the nucleotide homology from 97.7% to 99.9%；they were at the same large branch with American strain 4-17-03，7-16-23，4-17-25 and Japanese strain GF2020，with a close genetic distance；but they were at different large branch with American original strain Mebus，with a distant genetic relationship and the nucleotide homology from 98.1% to 98.2%. In conclusion，BCoV might be widely prevalent in the cattle population in Yunnan Province，especially the calves at 1 week to 1 month old，which might be caused by transportation or introduction. Therefore，it was necessary to strengthen the monitoring，control and quarantine against the virus，and speed up the development and application of corresponding vaccines.