In order to ensure the scientificity and accuracy of test results obtained by veterinary laboratories，relevant laboratories were organized by Pu'er Center for Animal Disease Prevention and Control to participate in a proficiency testing. From 2014 to 2021，44 comparison projects were implemented in the city. The results showed that the number of involved laboratories increased from 4 to 10；the average accuracy of the test results obtained by the laboratories under animal disease prevention and control institutions at municipal level，border animal disease monitoring points and the laboratories under animal disease prevention and control institutions at county（district）level were 97.3%，95.7% and 95.9%，respectively. In conclusion，the veterinary laboratories，through improving capacity building by examination，had achieved remarkable results with higher level of detection in general，which could support the risk assessment on animal diseases and the system of prevention and control. Considering poor capability for HA-HI test and pathogenic detection and other current shortcomings，relevant authorities were recommended to train laboratory staff with strong professional quality，increase inputs，accelerate to build a management system of veterinary laboratories，with a view to further improving the comprehensive level of veterinary laboratories.