CRISPR/Cas技术在动物疫病诊断中的应用
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S851.3

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Application of CRISPR/Cas in the Diagnosis of Animal Diseases
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    摘要:

    传统的动物疫病检测方法大多耗时长,操作复杂,对人员和仪器设备要求较高,不适用于基层动物疫病监测和初步筛查,因此开发灵敏度高、低成本、快速、便携,可实现现场诊断的病原检测新技术尤为重要。成簇规律间隔短回文重复序列及其相关蛋白(CRISPR/Cas)作为一种适应性免疫系统,因其简单、高效的基因编辑能力,已被广泛用于动物疫病、植物育种、人类医学等领域。近年来,CRISPR/Cas系统得到不断发展,已被开发为一种分子诊断工具,在动物疫病诊断领域显示出巨大潜力。本文简要介绍了CRISPR/Cas系统的基本结构、分类和作用机制,重点阐述了其在动物病毒病、细菌病及寄生虫病等诊断领域的应用研究进展,旨在为相关领域研究提供参考。

    Abstract:

    It is particularly important to develop a new technique with the advantages of high sensitivity,low cost,rapidity and portability for detecting pathogens in the field as most of the traditional detection methods for animal diseases are time-consuming,complex in operation,requiring more for operators and instruments,and are not suitable for animal disease monitoring and preliminary screening at field level. Clustered regularly interspaced short palindromic repeats/associated protein system(CRISPR/Cas),as an adaptive immune system,has been widely used in animal diseases,plant breeding,human medicine and other areas because of its simple and efficient gene editing capabilities. In recent years,CRISPR/Cas system has been continuously developed,and used as a molecular diagnostic tool with huge potential in the field of animal disease diagnosis. In the paper,the basic structure,classification and mechanism of CRISPR/Cas system were briefly introduced,especially its advances in the diagnosis of animal viral diseases,bacterial diseases and parasitic diseases,with a view to providing a reference for researches in related fields.

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尚佳静,罗娟,王帅,张富友,邓春冉,于晓慧,刘华雷,刘冠慧,李阳. CRISPR/Cas技术在动物疫病诊断中的应用[J].《中国动物检疫》编辑部,2022,39(11):80-88.

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  • 在线发布日期: 2022-11-03
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