In order to monitor the prevalence and genetic variation of porcine reproductive and respiratory syndrome virus（PRRSV）in Western Hubei，672 samples of blood and lung tissues collected from the suspiciously infected pigs in 8 counties（cities/districts）in the region in 2022 were detected for PRRSV by real-time fluorescent RT-PCR，followed by analysis on the genetic evolution of the ORF5 gene sequence of some positive samples. The results showed that 33 positive samples were detected，with a positive detection rate of 5%；after further typing and identification，23 were positive for HP-PRRSV，accounting for 69.7%，and 10 were positive for NADC30-like PRRSV，accounting for 30.3%；GP5 protein amino acid sequences of four positive samples were analyzed by DNAstar software，it was observed that multiple amino acid mutations were available，29G→29C appeared in the decoy epitope（27 to 30 aa），39A→39T in the neutralizing epitope of HP-PRRSV，and 38A→38T in the NADC30-like strain. In conclusion，two strains of PRRSV including the high pathogenic one and NADC30-like one were prevalent in western Hubei，and several variations had occurred in their GP5 protein amino acid sequences. Therefore，it was recommended to continuously strengthen molecular epidemiological surveillance for PRRSV，and to select appropriate vaccines corresponding to the epidemic strain.