2022年鄂西地区PRRSV ORF5基因遗传进化分析
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S852.65

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2022年度宜昌市科技研究与开发项目


Analysis on the Genetic Evolution of PRRSV ORF5 Gene in Western Hubei in 2022
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    摘要:

    为掌握鄂西地区PRRSV的流行及遗传变异情况,对2022年采自该地区8个县(市、区)的疑似感染发病猪血液、肺脏组织等样品共672份,进行PRRSV实时荧光RT-PCR检测,并对部分阳性样品的ORF5基因序列进行遗传进化分析。结果显示:检出PRRSV阳性样品33份,阳性检出率为5%;通过进一步分型鉴定,23份为HP-PRRSV阳性,占69.7%,10份为类NADC30(NADC30-like PRRSV)阳性,占30.3%;采用DNAstar软件分析4份阳性样品的GP5蛋白氨基酸序列,显示存在多个氨基酸突变,在诱骗表位(27~30 aa)出现29G→29C,在中和表位,HP-PRRSV毒株出现39A→39T,类NADC30毒株出现38A→38T。结果表明,鄂西地区存在高致病性和类NADC30两种PRRSV毒株流行,其GP5蛋白氨基酸序列已发生了多个变异。结果提示,应持续加强PRRSV分子流行病学监测,选择与流行毒株匹配的疫苗进行免疫。

    Abstract:

    In order to monitor the prevalence and genetic variation of porcine reproductive and respiratory syndrome virus(PRRSV)in Western Hubei,672 samples of blood and lung tissues collected from the suspiciously infected pigs in 8 counties(cities/districts)in the region in 2022 were detected for PRRSV by real-time fluorescent RT-PCR,followed by analysis on the genetic evolution of the ORF5 gene sequence of some positive samples. The results showed that 33 positive samples were detected,with a positive detection rate of 5%;after further typing and identification,23 were positive for HP-PRRSV,accounting for 69.7%,and 10 were positive for NADC30-like PRRSV,accounting for 30.3%;GP5 protein amino acid sequences of four positive samples were analyzed by DNAstar software,it was observed that multiple amino acid mutations were available,29G→29C appeared in the decoy epitope(27 to 30 aa),39A→39T in the neutralizing epitope of HP-PRRSV,and 38A→38T in the NADC30-like strain. In conclusion,two strains of PRRSV including the high pathogenic one and NADC30-like one were prevalent in western Hubei,and several variations had occurred in their GP5 protein amino acid sequences. Therefore,it was recommended to continuously strengthen molecular epidemiological surveillance for PRRSV,and to select appropriate vaccines corresponding to the epidemic strain.

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欧阳艳,刘发志,王孝忠,李祚丹,熊同舟,杜建丰.2022年鄂西地区PRRSV ORF5基因遗传进化分析[J].《中国动物检疫》编辑部,2022,39(12):12-17.

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  • 在线发布日期: 2022-12-05
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