A case of collective diarrhea was detected in a large-scale farm in Fujian Province. In order to identify the pathogen causing the disease and the status of infection，feces and oral swabs of pigs at different growth stages，as well as samples from environment in delivery rooms and personnel appearance（clothing and shoe soles）were collected for detecting the viral load by fluorescent quantitative PCR. The results showed that the disease was caused by porcine rotavirus（PoRV）that widely contaminated and was detected in all stages of pig population，the detection rate of personnel appearance was 87.50%，so it was likely to spread via the virus carried by personnel. The disease was controlled by a series of measures including vaccination，disinfection and management of feces，and no new case had occurred. A reference was therefore provided for scientific prevention and control of porcine rotavirus infection in farms.