2018—2021年甘肃省武威市羊布鲁氏菌S2疫苗免疫抗体监测
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S851.3

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甘肃省科技计划项目(20YF8NH153)


Surveillance on Immune Antibodies against S2 Vaccine Strain of Sheep Brucella in Wuwei City,Gansu Province from 2018 to 2021
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    摘要:

    为全面了解甘肃省武威市羊布鲁氏菌S2疫苗的口服免疫效果,掌握大群体免疫的抗体变化情况,2018—2021年,通过虎红平板凝集试验(RBPT)和试管凝集试验(SAT),对71个口服免疫S2疫苗的规模羊场户,采集8 243份羊血液样本进行免疫抗体检测。结果显示:口服免疫S2疫苗(1×1010 CFU剂量)后28 d的免疫抗体转阳率为55.62%,60 d为16.72%;2018—2021年,免疫后28 d的免疫抗体转阳性率为53.21%~57.91%,60 d为15.55%~17.81%,年度间无显著差异(P>0.05);天祝县免疫后28 d的免疫抗体转阳率最高,民勤县最低,差异显著(0.01<P<0.05),而其他县区间无显著性差异(P>0.05),县区间免疫后60 d的免疫抗体转阳率均无显著性差异(P>0.05);藏羊(59.04%)、高山细毛羊(58.06%)免疫后28 d的抗体转阳率较高,小尾寒羊最低(51.94%),差异显著(0.01<P<0.05),其他品种间均无显著性差异(P>0.05),免疫后60 d品种间均无显著性差异(P>0.05);免疫后28 d,存栏量小于100只和大于100只的羊场户抗体转阳率无显著性差异(P>0.05),而免疫后60 d差异极显著(P<0.01)。结果表明:武威市羊群S2疫苗口服免疫后的抗体转阳率总体不高,抗体存续时间较短;不同年度、不同县区的免疫效果基本一致;藏羊、高山细毛羊的免疫效果好于小尾寒羊,小规模羊群的免疫效果略好于大规模羊群。结果提示,布鲁氏菌病免疫需综合考虑大群免疫影响因素,探索适宜的疫苗免疫模式和免疫剂量,确保免疫覆盖率,以提升群体的整体免疫效果。

    Abstract:

    In order to evaluate the oral immunization effect of S2 vaccine for brucellosis in Wuwei City,Gansu Province,and to investigate the antibody changes of mass population immunization,from 2018 to 2021,8 243 sheep blood samples were collected from 71 large-scale farms where sheep was orally immunized with S2 vaccine for brucellosis,to detect immune antibodies by rose bengal plate test(RBPT)and standard-tube agglutination test(SAT). The results showed that the positive conversion rate was 55.62% 28 days and 16.72% 60 days after oral immunization with S2 vaccine(1×1010 CFU);for the three years,the positive conversion rate(the rate)was from 53.21% to 57.91% 28 days after immunization,and from15.55% to 17.81% after 60 days,and no significant difference was observed between years(P>0.05);the rate was the highest 28 days after vaccination in Tianzhu County and the lowest in Minqin County,with significant difference(0.01<P<0.05),but little among other counties/districts(P>0.05),and no significant difference was observed in the rate 60 days after vaccination among counties/districts(P>0.05);the rate was higher in Tibetan sheep(59.04%)and Alpine fine wool sheep(58.06%)28 days after vaccination,and lowest in small fat-tail sheep(51.94%),with significant difference(0.01<P<0.05),but little among other varieties(P>0.05). 60 days after vaccination,no significant difference was found among different varieties(P>0.05);and 28 days after vaccination,the rate was similar(P>0.05)in the farms with an inventory of less or more than 100 sheep,but extremely different 60 days after vaccination(P<0.01). In conclusion,the rate after immunization was relatively low in general,with low duration of antibodies;the immunization effect was basically consistent in different years and counties,and better in Tibetan sheep and Alpine fine wool sheep compared with small fat-tail sheep. It was therefore recommended to improve the immunity of the whole population through developing an appropriate model and dose of vaccine immunization to expand the coverage rate of immunization in consideration of the factors influencing mass immunization.

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王建军,张金学,方玉鹏,马金宝,董伟,李晓燕,魏晓燕.2018—2021年甘肃省武威市羊布鲁氏菌S2疫苗免疫抗体监测[J].《中国动物检疫》编辑部,2023,40(1):12-16.

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  • 在线发布日期: 2023-01-10
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