新疆部分焉耆马场马梨形虫病病原学检测及序列分析
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S852.7

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新疆维吾尔自治区自然科学基金面上项目(2022D01A65);新疆维吾尔自治区区域协同创新专项(2021E01001)


Pathogenic Detection and Sequence Analysis of Equine Piroplasmosis in Some Yanqi Horse Farms in Xinjiang
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    摘要:

    为了解新疆地区焉耆马场马梨形虫病病原感染情况,促进当地马梨形虫病的综合防控和马产业的健康发展,选取5个焉耆马场作为试验点,随机采集156匹焉耆马全血进行马梨形虫病病原DNA检测,并使用SPSS Statistics 17.0、Megalign与MEGA 11.0软件分析不同采样点、不同年龄、不同性别马匹的感染情况差异,同时进行病原核酸的同源性比较和系统进化树构建。结果显示:156匹焉耆马全血样品中,检出马梨形虫病病原核酸阳性82份,其中马泰勒虫、马驽巴贝斯虫和两者混合感染阳性率分别为33.3%(52/156)、26.9%(42/156)和7.7%(12/156)。不同采样点、不同年龄、不同性别焉耆马的马梨形虫病病原阳性率均无显著差异(P>0.05)。通过Megalign与MEGA11.0软件分析发现:测序得到的马泰勒虫18S rRNA基因序列与选取的参考虫株同源性为95.5%~100%,马驽巴贝斯虫BC-18S rRNA基因序列与选取的参考虫株同源性为98.6%~99.6%。马泰勒虫18S rRNA基因与马泰勒虫伊犁株(OL589505.1)位于同一分支上,进化关系较近,与其他地区马泰勒虫株进化关系较远;马驽巴贝斯虫BC-18S rRNA与马驽巴贝斯虫新疆株(MT563457.1)位于同一分支上,进化关系较近,与其他地区马驽巴贝斯虫株关系较远。结果表明,新疆地区焉耆马场的马梨形虫病病原感染情况较为严重,主要通过本地蜱叮咬传播,需要加强马梨形虫病的监测与防控,做好定期驱蜱工作。本调查为当地马梨形虫病的综合防控提供了一线数据。

    Abstract:

    In order to investigate the infection status of equine piroplasmosis in Yanqi horse farms in Xinjiang,and to implement comprehensive prevention and control measures to ensure healthy development of horse industry in the region,five farms were selected to collect blood samples from 156 Yanqi horses for DNA detection of equine piroplasmosis pathogen. The infection status of the horses at different ages and in different sampling sites and of different genders was analyzed by SPSS Statistics 17.0,Meaglign and MEGA 11.0,meanwhile,the homology of pathogenic nucleic acids was compared and a phylogenetic tree was constructed. The results showed that 82 positive samples for equine piroplasmosis pathogen nucleic acids were detected,the positive rates of Theileria equi(T. equi),Babesia caballi(B. caballi)and their mixed infection were 33.3%(52/156),26.9%(42/156)and 7.7%(12/156),respectively. There was no significant difference(P>0.05)in the positive rate of equine piroplasmosis in different sampling sites and the horses at different ages and of different genders. It was found that,by the analysis with Meaglign and MEGA11.0,the homologies of the obtained gene sequences of T. equi 18S rDNA and B. caballi BC-18S rRNA were from 95.5% to 100% and from 98.6% to 99.6%,respectively with the reference strain. For T. equi,its 18S rRNA gene and Ili strain(OL589505.1),with closer evolutionary relationship,were located in the same branch,but far from the strains in other regions,so was the BC-18S rRNA gene of B. caballi and Xinjiang strain(MT563457.1). In conclusion,the disease was widely prevalent in Xinjiang,which should be further monitored,prevented and controlled. Field data was therefore provided by the investigation described in the paper for future comprehensive prevention and control of the disease.

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诺明达来,甘露,李丽,刘燕,呼尔查,巴音查汗.新疆部分焉耆马场马梨形虫病病原学检测及序列分析[J].《中国动物检疫》编辑部,2023,40(1):17-21.

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