一起牦牛呼吸道疾病的病原学诊断
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S852.65

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“十四五”国家重点研发计划项目(2021YFD1600203);四川省高等学校重点实验室——动物医学实验室项目(2021PTJS34);国家农业产业技术体系四川肉牛创新团队专项(SCCXTD-2020-13)


Etiological Diagnosis on a Case of Yak Respiratory Disease
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    摘要:

    2022年2月,川西北某地牦牛暴发以呼吸道症状为突出特征的传染病。为确定病因,对发病牦牛进行了相关病原的病原学诊断。共采集13头发病牦牛的20份病料,采用PCR方法对牛病毒性腹泻病毒(BVDV)、牛副流感病毒3型(BPIV-3)、牛传染性鼻气管炎病毒(IBRV)、牛冠状病毒(BCoV)、牛呼吸道合胞体病毒(BRSV)、牛腺病毒3型(BAdV-3)以及多杀性巴氏杆菌(P. multocida,Pm)、溶血性曼氏杆菌(M. haemolytica,Mh)和牛支原体(M. bovis,Mb)等9种牛呼吸道病原进行检测。结果显示:在20份样本中共检测出IBRV、BAdV-3和Pm 3种病原,且存在混合感染,其他病原未检出;4份全血样本中检测出3份IBRV、1份BAdV-3阳性,1份胎盘组织样本中检出IBRV阳性,8份鼻拭子样本中检出3份IBRV、5份BAdV-3阳性,2份流产中阴道拭子中检出2份BAdV-3阳性,5份肺脏组织样本中检出4份Pm阳性;进一步对IBRV和Pm进行分型鉴定,确定该地牦牛感染的为1.2型IBRV和A型Pm。结果表明,引发该地牦牛呼吸道疫病的是1.2型IBRV、BAdV-3和A型Pm。本研究证实了BAdV-3与1.2型IBRV在该地牦牛中的存在和流行,为当地牦牛呼吸道疾病防控提供了参考。

    Abstract:

    In February 2022,an infectious disease with respiratory symptoms was detected in yaks in northwest Sichuan Province. In order to identify the etiology leading to the disease,an etiological diagnosis for related pathogens was carried out. A total of 20 lesions from 13 infected yaks were collected to detect 9 bovine respiratory pathogens including bovine viral diarrhea virus(BVDV),bovine parainfluenza virus type 3(BPIV-3),bovine infectious rhinotracheitis virus(IBRV),bovine coronavirus(BCoV),bovine respiratory syncytial virus(BRSV),bovine adenovirus type 3(BAdV-3),Pasteurellus multitolutoides(P. multocida,Pm),M. haemolytica(Mh)and M. bovis(Mb)by PCR. The results showed that IBRV,BAdV-3 and Pm,even co-infection,were detected from 20 samples,but no other pathogens were observed;the following viruses were detected:IBRV from 3 and BAdV-3 from 1 out of 4 whole blood samples;IBRV from 1 placental tissue sample and 3 out of 8 nasal swab samples,BAdV-3 from 5 nasal swab samples and 2 abortion swabs,and Pm from 4 out of 5 lung tissue samples;it was determined that,through further typing and identification of IBRV and Pm,the yaks were infected with IBRV 1.2 type and Pm A type. In conclusion,the respiratory disease prevalent in the region was caused by IBRV 1.2 type,BAdV-3 and Pm A type. It was confirmed by the study that BAdV-3 and IBRV 1.2 type were available and prevalent in yaks in the region,providing a reference for the prevention and control of respiratory diseases in local yaks.

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蒋娇娇,岳华,汤承.一起牦牛呼吸道疾病的病原学诊断[J].《中国动物检疫》编辑部,2023,40(1):29-34.

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  • 在线发布日期: 2023-01-10
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