eDNA技术在水生动物疫病监测中的适用性
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S851.3

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南京海关科研项目(2022KJ17)


Applicability of eDNA Technology in Aquatic Animal Disease Surveillance
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    摘要:

    使用环境DNA(environmental DNA,eDNA)监测水生系统是一个快速发展的研究方向。eDNA技术采用快速、经济、非损伤性采样方案,更适用于稀有、珍贵的水生动物,或难以收集的野生动物的疫病检测和监测。本文结合世界动物卫生组织(WOAH)水生动物委员会(AAC)讨论性文件,综述了eDNA技术在水生动物疫病监测中样品的收集和处理、方法的验证与确认以及在监测方面的适用性等;提出了eDNA方法无法获得诊断性能的准确评估,不适合用来支持WOAH名录中疫病的无疫声明,但阳性结果可能是可疑病例的适当判定标准等。未来可发挥其优势,进一步尝试将eDNA方法用于出入境食用、种用和观赏用水生动物养殖场、隔离暂养场等水生动物疫病监测,特别是特种高货值水生动物及大型水面水生动物疫病的监测。

    Abstract:

    It is a research direction under rapid development to monitor aquatic systems by environmental DNA(eDNA)that,with rapid,economic and non-invasive sampling plan,is more suitable for disease detection and monitoring of rare or precious aquatic animals or wild animals that were difficult to be sampled. In the paper,based on the documents for discussion issued by Aquatic Animal Committee(AAC),World Organization for Animal Health(WOAH),the collection and disposal of samples in aquatic animal disease surveillance by eDNA,the validation and confirmation of the method,and its applicability in the surveillance were reviewed,and it was proposed that the positive result might be an appropriate criteria to determine any suspicious cases,although eDNA was not suitable to support any free declaration of WOAH listed animal diseases because it failed to obtain any accurate estimates of diagnostic performance. It was recommended that,with its advantages,eDNA could be used in aquatic animal disease surveillance in entry-exit establishments and temporary quarantine facilities holding aquatic animals intended for human consumption,breeding and ornamental purposes,especially used in the surveillance on the diseases of special high-value aquatic animals and aquatic animals living on large water surfaces.

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段宏安,徐晔,王凤芝,周毅,丁超. eDNA技术在水生动物疫病监测中的适用性[J].《中国动物检疫》编辑部,2023,40(1):80-87.

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  • 在线发布日期: 2023-01-10
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