犬瘟热、犬细小病毒胶体金二联检测卡研制
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

S855.3

基金项目:

犬疫病快速诊断及防控关键技术研究与应用项目(20180323)


Development of Colloidal Gold-based Dual Detection Card for Canine Distemper Virus and Canine Parvovirus
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    本研究通过细胞融合、间接ELISA方法筛选出4株可稳定分泌犬瘟热病毒(canine distemper virus,CDV)和犬细小病毒(canine parvovirus,CPV)单克隆抗体的杂交瘤细胞株,以此为基础研制了一种可同时检测CDV和CPV的胶体金二联检测卡,并对其特异性、灵敏度及准确性进行了测试。结果显示:4株杂交瘤细胞可稳定传代,分泌的单克隆抗体纯度高,效价均在1:320以上。制备的胶体金二联检测卡特异性强,只与CDV和CPV产生特异性条带,而不与其他犬类病毒反应;灵敏度高,最低检测限可达到102拷贝/μL;准确性好,与荧光定量PCR结果的符合率高于88.0%,与市面上单一病毒检测卡的符合率高于97.2%。结果表明,本研究制备的CDV、CPV胶体金二联检测卡特异性强、灵敏度高、准确性好,兼具操作简便、肉眼可判读、结果易保存和无需特殊仪器设备等优势,可用于两种疾病的临床快速诊断和大规模检测工作。

    Abstract:

    Four strains of hybridomas that could stably secrete monoclonal antibodies against canine distemper virus(CDV)and canine parvovirus(CPV)were screened by cell fusion and indirect ELISA,based on which,a colloidal gold-based dual detection card that could simultaneously detect CDV and CPV was developed,then its specificity,sensitivity and accuracy were tested. The results showed that the four strains of hybridomas could be stably passaged,the purity of monoclonal antibodies secreted was high,and all the titers were above 1:320;the prepared card,with strong specificity,could produce specific strips with CDV and CPV only,but failed with other canine viruses;high sensitivity was observed,and the minimum detection limit could reach 102 copies/μL;and good accuracy made the coincidence rate with the results by fluorescent quantitative PCR higher than 88.0%,and that with the single virus detection card higher than 97.2%. In conclusion,the prepared card was with high sensitivity,specificity and accuracy as well as other advantages such as simple operation,visual interpretation,easy preservation of results,and working without any special instruments and equipment,which could be used for rapid clinical diagnosis and massive detection of the two diseases.

    参考文献
    相似文献
    引证文献
引用本文

余春明,郑雪莹,范君文,史记,李华斌,郑金来,邓柏林.犬瘟热、犬细小病毒胶体金二联检测卡研制[J].《中国动物检疫》编辑部,2023,40(1):128-133.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2023-01-10
  • 出版日期:
您是第位访问者
《中国动物检疫》编辑部 ® 2023 版权所有
地址:山东省青岛市南京路369号《中国动物检疫》编辑部 邮编:266032
电话:0532-85623545 0532-85622559 0532-85642906 传真:0532-85621826 鲁ICP备08016398号-3
技术支持:北京勤云科技发展有限公司