为评估不同免疫剂量禽流感灭活疫苗对父母代蛋种鸡产蛋率及抗体效价的影响，在河北省某海兰褐蛋种鸡场挑选产蛋稳定的720羽55周龄蛋种鸡，平均分为6组，每组4个重复，每个重复30羽；试验组分别胸部皮下注射0.2、0.3、0.5、0.7 mL/羽的重组禽流感病毒H5+H7三价灭活疫苗（H5N1 Re-11株+Re-12株，H7N9 Re-3株），同时设0.5 mL生理盐水组及不免疫空白对照组；统计免疫前及免疫后1~4周各组的产蛋率，并分别在免疫前及免疫后3、6、10、14周采血，检测其抗体水平。结果显示：免疫后2周，0.7 mL组产蛋率下降幅度最大，与对照组差异极显著（P＜0.01），而0.2 mL组下降幅度最小，与对照组差异不显著（P＞0.05）。各免疫组免疫后3周的H5N1 Re-11抗体水平与免疫前相比均显著上升（P＜0.05），免疫后14周0.2 mL组与0.7 mL组抗体滴度最高，均为8.60log2；免疫后3周的H7N9 H7-Re3抗体水平与对照组相比均显著上升（P＜0.05），且各免疫组间的上升幅度差异均不显著（P＞0.05），免疫后14周，0.7、0.3、0.2 mL组的抗体滴度较高，均在7.60log2以上。结果表明，0.2 mL/羽的免疫剂量对蛋鸡产蛋性能影响最小，且诱导的抗体水平及其维持时间均与0.7 mL/羽相当，适于作为产蛋期禽流感的最佳免疫剂量。本研究为养鸡场产蛋期禽流感最适加强免疫剂量的选择提供了依据。
In order to evaluate the effect of inactivated avian influenza vaccines at different doses on laying rate and antibody titer of parental layer breeders，720 55-week-old layer breeders with regular production were selected from a farm with Hy-line Variety Brown in Hebei Province and divided into 6 groups on average，each of which with 4 repeats and 30 layer breeders per repeat；for the test group，the recombinant avian influenza virus H5+H7 trivalent inactivated vaccines（H5N1 Re-11 strain + Re-12 strain，H7N9 Re-3 strain）at the doses of 0.2，0.3，0.5 and 0.7 mL/layer breeder were respectively injected subcutaneously into each chest respectively，meanwhile，0.5 mL normal saline group and non-immune blank control group were set；the laying rates were calculated before vaccination and at 1，2，3 and 4 weeks after vaccination，and blood samples were collected before vaccination and at 3，6，10 and 14 weeks after vaccination to detect antibody levels. The results showed that，at 2 weeks after vaccination，the laying rate of the group injected with 0.7 mL decreased the most compared with the control group，the difference was extremely significant（P＜0.01），while that with 0.2 mL decreased the least，with no significant difference（P＞0.05）. The antibody titers against H5N1 Re-11 strain in each group at 3 weeks after vaccination were significantly higher than those before vaccination（P＜0.05）. At 14 weeks after vaccination，the antibody titers against H5N1 Re-11 strain in the group injected with 0.2 mL or 0.7 mL were the highest，and both of which were 8.60log2；at 3 weeks after vaccination，the antibody titers against H7N9 H7-Re-3 strain were significantly higher than those in the control group（P＜0.05），the range of increase in each group with vaccination was slightly different（P＞0.05），at 14 weeks after vaccination，all the antibody titers of the groups with 0.7，0.3 and 0.2 mL were above 7.60log2. In conclusion，the dose of 0.2 mL/layer breeder could be used as the optimal one for avian influenza during the laying period，as the performance of layer breeders was influenced the least，the induced antibody level and its maintenance time were equivalent to those of 0.7 mL. A reference was therefore provided by the study for selecting the optimal booster dose for avian influenza during the laying period in the farms.