In order to evaluate the effect of inactivated avian influenza vaccines at different doses on laying rate and antibody titer of parental layer breeders，720 55-week-old layer breeders with regular production were selected from a farm with Hy-line Variety Brown in Hebei Province and divided into 6 groups on average，each of which with 4 repeats and 30 layer breeders per repeat；for the test group，the recombinant avian influenza virus H5+H7 trivalent inactivated vaccines（H5N1 Re-11 strain + Re-12 strain，H7N9 Re-3 strain）at the doses of 0.2，0.3，0.5 and 0.7 mL/layer breeder were respectively injected subcutaneously into each chest respectively，meanwhile，0.5 mL normal saline group and non-immune blank control group were set；the laying rates were calculated before vaccination and at 1，2，3 and 4 weeks after vaccination，and blood samples were collected before vaccination and at 3，6，10 and 14 weeks after vaccination to detect antibody levels. The results showed that，at 2 weeks after vaccination，the laying rate of the group injected with 0.7 mL decreased the most compared with the control group，the difference was extremely significant（P＜0.01），while that with 0.2 mL decreased the least，with no significant difference（P＞0.05）. The antibody titers against H5N1 Re-11 strain in each group at 3 weeks after vaccination were significantly higher than those before vaccination（P＜0.05）. At 14 weeks after vaccination，the antibody titers against H5N1 Re-11 strain in the group injected with 0.2 mL or 0.7 mL were the highest，and both of which were 8.60log2；at 3 weeks after vaccination，the antibody titers against H7N9 H7-Re-3 strain were significantly higher than those in the control group（P＜0.05），the range of increase in each group with vaccination was slightly different（P＞0.05），at 14 weeks after vaccination，all the antibody titers of the groups with 0.7，0.3 and 0.2 mL were above 7.60log2. In conclusion，the dose of 0.2 mL/layer breeder could be used as the optimal one for avian influenza during the laying period，as the performance of layer breeders was influenced the least，the induced antibody level and its maintenance time were equivalent to those of 0.7 mL. A reference was therefore provided by the study for selecting the optimal booster dose for avian influenza during the laying period in the farms.