In order to investigate the infection with pseudorabies virus（PRV）in swine breeding farms in Beijing City，10 850 serum samples collected from 3 districts from 2019 to 2021 were detected for antibodies against PRV（gE antibodies）by enzyme-linked immunosorbent assay（ELISA）. The results showed that the population apparent positive rates of the farms for three consecutive years were 84.38%，77.78% and 64.71% respectively，and the true ones were 86.10%（from 73.95% to 98.24%），79.37%（from 60.39% to 98.34%）and 66.03%（from 43.22% to 88.83%），respectively，with a slightly downtrend（P＞0.05）；the individual apparent positive rates were 53.95%，41.52% and 35.59%，respectively，and the true ones were 55.05%（from 53.05% to 57.05%），42.37%（from 40.90% to 43.84%）and 36.32%（from 34.85% to 37.79%）respectively，with a significant downtrend（P＜0.05）. The individual positive rates of growers，multiparous sows and reserve sows decreased year by year，while that of fattening pigs increased from 38.45% in 2019 to 60.79% in 2021. In conclusion，PRV infection was controlled in the breeding farms to some extent，although still serious，especially in fattening pigs，which should continue to strengthen immunization，monitoring and construction of a biosafety system，and to gradually achieve the goal of purification and eradication of PRV in farms.