In order to preliminarily investigate the antibody level of three major zoonoses including rabies，toxoplasmosis and brucellosis prevalent in Dongguan City，Guangdong Province，1 303 serum samples collected from dogs and cats（1 174 from dogs and 129 from cats）in 32 towns（sub-districts）in the city were serologically tested by enzyme-linked immunosorbent assay（ELISA）. The results showed that the positive rate of rabies immune antibodies in dogs and cats was 80.74%，Toxoplasma gondii antibodies were detected only in dogs，with the positive rate of 0.85%，and no Brucella-positive sample was detected. The positive rate of rabies antibodies in pet dogs and cats was significantly higher than that in the native ones（P＜0.01），that in dogs and cats aged over 3 years old was significantly higher than that in those at or under 3 years old（P＜0.05）；the positive rate of Toxoplasma gondii antibodies in native dogs was significantly higher than that in pet dogs（P＜0.05），and that in the dogs under 1 year old was significantly higher than that in those over 1 year old. In conclusion，the positive rate of rabies immune antibodies in dogs and cats in Dongguan was high with good immune effect，although a potential risk of Toxoplasma gondii infection was available；the positive rate might be influenced by feeding modes and ages of dogs and cats，against which，intensive monitoring should be conducted，and target measures should be applied according to the results of monitoring. Current antibody level of the three major zoonoses was identified by the investigation，which provided a reference for further development of corresponding prevention and control measures.