In order to rapidly detect Maedi-visna virus（MVV），specific primers and probes were designed according to gag gene of MVV conserved sequence，followed by optimization of the reaction conditions and reaction system，a real-time fluorescent RPA for MVV was established and evaluated through the tests of sensitivity，specificity，repeatability and sample recheck. The results showed that，by the established method，the tests could be finished within 20 min at an optimal reaction temperature of 39 ℃；its lowest detection limit could reach 10-1 pg/μL with high sensitivity，which was 104 times more sensitive than the routine PCR；failed to crossly react with Peste des petits ruminants virus（PPRV），Streptococcus septicaemia（OSS），bovine enterovirus （BEV），infectious rhinotracheitis virus（IBRV），bovine parainfluenza virus type 3（BPIV3），Mycoplasma pneumoniae（MO）and other pathogens with secondary respiratory symptoms due to its strong specificity；the coefficient of variation（CV）for detecting template DNA with the same mass concentration was less than 10% due to its good repeatability；MVV could be effectively detected，which was consistent with the results by PCR. In conclusion，the established method could be used to rapidly detect MVV in practice，and supported the diagnosis，prevention and control of the disease.