为探究当前我国常用的布鲁氏菌病活疫苗（M5、S2株）对羔羊免疫后血清抗体产生以及早期生产性能的影响，将90只1月龄湖羊羔羊随机均分为3组（M5免疫组、S2免疫组、对照组），M5免疫组皮下注射M5株疫苗，S2免疫组口服S2株疫苗，对照组肌内注射生理盐水。免疫后观察疫苗免疫副反应，并于免疫前及免疫后7、14、21、28、90、120 d采集血液样品进行抗体检测，首先通过虎红平板凝集试验（RBT）初检，再采用试管凝集试验（SAT）复核。参试羊群于免疫前及免疫后30、60、90、120 d称重，分析判断M5、S2株疫苗对羔羊生长性能的影响。结果显示：M5株疫苗免疫羔羊引起的免疫副反应较大，S2株疫苗相对较小；M5株疫苗免疫后7 d即可检测到血清抗体，血清抗体阳性率为42.86%，14 d达到峰值（96.43%），120 d降至29.63%；S2株疫苗免疫羔羊后7 d同样可检测到血清抗体，阳性率为34.48%，14 d血清抗体阳性率达93.10%，120 d降至27.59%。免疫M5、S2株疫苗均会对羔羊早期生长发育产生影响，导致生长发育缓慢，其中M5株疫苗影响较大，羔羊在各阶段的体重指标以及阶段总增重指标均极显著低于对照组（P＜0.01）；S2株疫苗免疫后，羔羊仅在3、4月龄体重指标和阶段总增重指标极显著低于对照组（P＜0.01），之后其生长性能有所恢复。本研究为制定科学合理的羔羊布鲁氏菌病疫苗免疫方案提供了数据支撑。
In order to identify the influence of live brucellosis vaccines（M5 and S2 strain）commonly used in China on the production of serum antibodies after immunization and growth of lambs，90 lambs of Hu sheep at one month old were randomly divided into three groups（M5 group，S2 group and the control group），M5 group was subcutaneously injected with M5 vaccine，S2 group was orally immunized with S2 vaccine，and the control group was intramuscularly injected with saline. All side effects after immunization were observed，and blood samples were collected before immunization and 7，14，21，28，90 and 120 days after immunization to detect antibodies by rose bengal plate agglutination test（RBT）followed by standard-tube agglutination test（SAT）for review. The lambs were weighed before immunization and 30，60，90 and 120 days after immunization to analyze and determine the influence of M5 and S2 on the lambs' growth. The results showed that more side effects could be caused by M5 as compared with S2；serum antibodies could be detected 7 days after immunization with M5，with a positive rate of 42.86%，reached a peak value（96.43%）after 14 days，then reduced to 29.63% after 120 days；for S2 vaccine，serum antibodies could also be detected 7 days after immunization，with a positive rate of 34.48%，reached to 93.10% after 14 days，then reduced to 27.59% after 120 days. The growth performance of the lambs at early stage could be delayed by M5 and S2 vaccine，especially M5 vaccine，for which，both the indexes of body weight and total weight gain were extremely significantly lower than those of the control group（P＜0.01）；while the indexes of the lambs only at 3 and 4 months old were extremely significantly lower than those of the control group（P＜0.01）when immunized with S2 vaccine，after that，the growth performance was gradually recovered. The development of a scientific and reasonable immunization plan for brucellosis in lambs was supported with data provided in the paper.