In order to establish an accurate assay for meat products derived from animals，a specific PCR assay was developed based on the specific genes of pigs，cattle，horses，goats，mice，rats，rabbits，dogs，chickens，ducks and geese using a bioinformatics method，which，with high specificity，could detect meat samples from pigs，cattle，horses，goats，rabbits，dogs，chickens，ducks and geese，and identify prohibited meat ingredients derived from rats，mice and others，with the minimum detection limit of 1.00 ng/μL of genomic DNA and 1% meat addition. 10 samples randomly collected from each 3 kinds of primarily processed meat products in markets were detected by the established method，it was found that adulterated samples were detected from all products，with an adulteration rate ranging from 10% to 20%. In conclusion，the quality testing of meat products in markets could be technically supported by the established method that was with the advantages of high sensitivity，accuracy，low cost and wide range of applicability.