曲径 , 江育林,沈海平,王浩,刘荭,连建华,潘雁甲,郑雪莲
1998(2):1-3.
摘要:Lymphocystis disease of Paralichtys olivaceus is an infectious disease of fish caused by lymphocystis virus (LCV) of Iridoviridae.The lymphocystis virus has a wide range of hosts causing serious losses. Outbreaks of the disease occurred in six fish farms. There were distinct cysts on the surface of the infected fish body. The size of the biggest cyst was about 500um in diameter. It was the first report ot the disease in China, and the disease was suspected to be introduced by importation of fishes from other country.The integrated conrtol measures were recommended to control the epidemic and prevent it from spreading and wipe it out as soon as possible.
1998(2):3-5.
摘要:A polymerase chain reaction (PCR)based method for the identification of swine meat by amplification of swine microsatellite DNA was established.The method not was only able to amplify raw swine DNA,but also cooked or autoclaved meat DNA.The sequence selected for amplification consisted of a 307 bp DNA fragment lying in the microsatellite DNA of swine.The specificity test demonstrated that this method was positive for raw swine meat, cooked and auto claved swine meat DNA ,but negative for equine,sheep,bovine,camel meat DNA .At least 38.6 fg of DNA from raw swine samples and 0.375 pg of DNA from cooked or autoclaved swine meat samples were detected respectively by PCR. We tested 63 swine samples by PCR and found a rate of correct identification up to 100%.The method needed only 6h for detection of meat products of all kinds.The results showed that the PCR method was sensitive,specific,convenient and rapid,so it may be suitable for rapid identification of swine meat.
1998(2):8-9.
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1998(2):14-16.
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1998(2):23-24.
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1998(2):24-25.
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1998(2):27-28.
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1998(2):37-37.
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1998(2):42-44.
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