1998(5):1-3.
摘要:A pair of primers were designed and synthesized based on Jean Dupouy-Camet 1.7-kb DNA sequence and used to detect larvae of T.isolates. They were isolated from pigs of Harbin Hailun, Shenyang,Henan Nanyang,Hubei Shiyan,XiAn,Yunnan Dali and dogs of Harbin Wuchang,Changchun and cats of Heilongjiang Sunwu respectively. The amplified products were analysed by agarose gel electrophoresis and the specific 602 and 230 bp bands shown in the isolates of larvae T.spiralis from pig, while no band appeared in isolates from dog and cat and normol mice.The parasite density detected by this method could be as low as 1 parasite, suggesting that PCR techniqe has high sensitivity and specificity.
1998(5):3-5.
摘要:One viral disease of Trionyx sinensis had been investigated in 4 culture regions in Fujian province by using epidemiological investigation and ultrathin section of the transmission electron microscope.The spherovirus infecting hepatocyte was the major pathogen which make a lot deaths in young turtles. The virus's ultrastructure and damaging effect to host cells were also been discussed.
1998(5):7-8.
摘要:
1998(5):9-10.
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1998(5):10-11.
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1998(5):23-24.
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1998(5):32-32.
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1998(5):36-37.
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1998(5):37-38.
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1998(5):41-42.
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