- 2002年第19卷第4期文章目次
>政策法规
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2002, 19(4):1-3.
摘要:
>防疫管理
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2002, 19(4):11-12.
摘要:
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2002, 19(4):12-12.
摘要:
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2002, 19(4):15-16.
摘要:
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2002, 19(4):19-20.
摘要:
>试验研究
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2002, 19(4):21-22.
摘要:A pair of primers were designed and synthesized based on the publised bovine PrPc protein gene sequense and Bam HI and Nde were added to the two ends of the primers respectively. A target gene fragment about 640 bp was amplified by PCR using the genosome DNA extracted from Chinese yellow cattle liver as template and cloned into plasmid pETlla , thus constructing PrPc protein gene recombinant plasmid b-pETlla-PrPc, transformed into DH-5a and sequenced. Homegenetic analysis showed that there are considerable differences between PrPc mature protein gene of Chinese yellow cattle and the published bovine PrPc mature protein gene abroad: a new Nde I restriction site was found; there were four amino acids variation in the derived amino acid sequences and lacked an eight amino acid repeated zone.
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2002, 19(4):23-24.
摘要:The recombinant plastid b-pETlla-PrPc of PrPc mature protein gene of Chinese yellow cattle was transformed into E.coi BL21(DE3)for induced expression. SDS-PAGE revealed that the molecular size of the expressed product was about 23KD, and identical to the expected value. West-blotting test confirmed the successful expression of PrPc mature protein of Chinese yellow cattle and it shared same antigen component as E.coli BL 21(DE3).
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2002, 19(4):24-25.
摘要:
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2002, 19(4):25-27.
摘要:
>防检技术
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2002, 19(4):28-29.
摘要:
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2002, 19(4):31-31.
摘要:
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2002, 19(4):32-32.
摘要:
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2002, 19(4):36-37.
摘要:
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2002, 19(4):38-39.
摘要:
>专论综述
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