2003, 20(11):9-9.
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2003, 20(11):10-11.
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2003, 20(11):13-14.
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2003, 20(11):15-16.
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2003, 20(11):19-19.
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2003, 20(11):21-22.
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张喜悦 , 徐天刚 , 王志亮 , 吴延功 , 王君玮 , 赵云玲 , 徐培莲
2003, 20(11):23-24.
摘要:OPD (O - phenylenediamine), TMB (tetramethylbenzidine) and ABTS (2,2' - Azino - bis - (3 - ethylben-zthiazoline- 6- sulfonic acid)) were used as substrates in ELISA with SI, S2 and S3 as Stopping solutions, for detection of bovine brucellosis in order to search suitable substrate. The results indicated that TMB was more proper as ELISA substrate.
2003, 20(11):27-29.
摘要:To establish a more rapid and simple method of detecting antibody of SFV, a dot immunogold filtration assay (DIGFA) was developed by coating the antigen of CSFV on nitrocellulose (NC) membrane, and labeling staphylococcal protein A (SPA) with colloidal gold.The color of the positive dots were so bright that the results could be easily determined.The whole procedure could be finished in 5 min.The method had a better specificity without cross - reaction with positive sera to PKRSV, PRV, PPV, NDV and AFV.Its coincidence with Dot - EUSA,indirect haemagglutination test(IH) were 98.4% and 98.9% respectively.DIGFA,more simple and rapid,was applicable to small diagnostic laboratories.lt could be applied for more early diagnosis ^ epidemiological investigation and the detection of antibody level in vaccinated herds.
2003, 20(11):30-31.
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2003, 20(11):36-37.
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2003, 20(11):37-37.
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2003, 20(11):42-43.
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