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摘要:An avian influenza virus(AIV) isolate A/Chicken/Wangcheng/4/2001(H9 N2)(WC2001)was propagated in specific pathogen free(SPF) chicken embryo. Base d on published gene sequence data, a pair of primers specific for NP gene was de signed. The NP cDNA was amplified by reverse transcription-polymerase chain rea ction(RT-PCR),then cloned and sequenced. The result showed that the cloned 1500 bp fragment covered the NP gene of the open reading frame encoding 496 amino aci ds of the NP gene. The NP gene sequence of WC2001 isolate was highly homologous to that of A/Hong Kong/Y280/97(H9N2).However, there were obvious diversities e xisted in the NP sequences among WC2001,2 human influenza virus isolates and 2 s wine influenza virus isolates.The WC2001 isolate demonstrated obvious gene chara cteristics of the avian influenza A virus.

摘要:A test paper for rapid diagnosis of IBD was developed by using gold immunochromatography assay.The test paper consisted of a sample pad,a conjugate reagent pad and a test pad.An IBD test can be finished shortly without any speci al instruments.In addition,the test paper had many virtues such as convenience, speediness, sensitivity and so on.

摘要:To avoid BSE transmitting into China, it is necessary to distinguish the animal species in meat and bone meal (MBM). Four of pairs primers were desi gned specific for the mitochondrial DNA sequences of bovine, ovine, porcine and chicken, and A species-specific PCR assay was established for the identificatio n of bovine, ovine, porcine and chicken species in MBM. After purification of th e template DNA from the MBM or processed meat tissue using Genomic DNA Purificat ion Kit, the specific amplification reactions were carried out. The products sho wed species-specific were 271bp, 199bp, 196bp, 148bp respectively, there were n o cross-reaction among them. The PCR assay was very useful as a rapid, sensitiv e, cheap and specific method for the routine screening of animal species in MBM.

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