2005, 22(10):20-22.
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2005, 22(10):23-26.
摘要:According to the sequence of ATCC VR-2332 strain of porcine reproductive and respiratory syndrome virus published by the Genbank, a pair of primers was designed for RT-PCR amplification of PRRSV isolates from Fujian,Zhejiang and Shandong, obtaining DNA fragment of about 748bp, which were cloned into pMD18-T vector respectively and sequenced. The sequences of ORF5 genes were analysed using DNA Star and compared with those of ATCC VR-2332,CH-la,MLV and LV strains,indicating that the homology was up-to 98.5 between SHD1 and FJ14, MLV, ATCC VR2332, 91.0% between SHD1 and CH-la and 86.6%-88.4% between SHD1 and other strains; 99.3%-99.7% between FJ14 and MLV,ATCC VR-2332.And the other isolates and CH-la were two distinct genetically. It is suggested that recent isolates of PRRSV are becoming move different from CH-la genetically.
2005, 22(10):27-29.
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2005, 22(10):30-31.
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2005, 22(10):32-32.
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2005, 22(10):33-33.
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2005, 22(10):38-38.
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2005, 22(10):45-45.
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2005, 22(10):46-47.
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