2005, 22(5):3-5.
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2005, 22(5):6-7.
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2005, 22(5):10-12.
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2005, 22(5):13-14.
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2005, 22(5):19-20.
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2005, 22(5):24-25.
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2005, 22(5):25-27.
摘要:According to the Genebank published sequence of west nile virus ,a pair of primers were designed and synthesized in order to amplify E gene of West Nile virus.The E gene was obtained by reverse transcript polymerase chain reaction(RT-PCR) ,subsequently cloned into pMD18-T-Vector.The nucleotide of West Nile virus E gene was compared with the corresponding sequences of published sequence of WNV,the nucleotide homology was 99.7% suggesting,that it is E gene of West Nile virus..
2005, 22(5):28-30.
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2005, 22(5):31-33.
摘要:An avian influenza virus (AIV) was isolated from a duck flock without any sign of clinical disease in early 2004, and named as A/Duck/HN/4/2004(H6N2). Squencing of the HA fragment showed that the full length of HA gene is 1744bp,covering the HA gene of the open reading frame,encoding 566 amino acid ,including 344 amino acid of HA1 and 221 amino acid of HA2.Compared with the same subtype sequence from GenBank ,their nucleotide homology of hemagglutinin was from 89.2% to 97.1% with H6 subtype hemmagglutinin.The phylogenetic relationships showed that the A/Duck/HN/4/2004(H6N2) was closest to the isolates (H6N2) from HongKong(A/Duck/HongKong/3600/99(H6N2)?A/Duck/HongKong/3600/99(H6N2)).
2005, 22(5):39-39.
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2005, 22(5):43-44.
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2005, 22(5):50-51.
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2005, 22(5):52-52.
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