- 2006年第23卷第3期文章目次
>政策法规
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2006, 23(3):5-6.
摘要:
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2006, 23(3):8-8.
摘要:
>防疫管理
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2006, 23(3):14-16.
摘要:
>肉品安全
>行业报道
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2006, 23(3):20-23.
摘要:
>试验研究
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祁会彩 , 龚振华 , 杨增岐 , 郑增忍 , 潘康锁 , 李葳 , 黄秀梅 , 郭福生 , 蒋正军
2006, 23(3):24-26.
摘要:A multiplex RT-PCR was optimized to simultaneously detect foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV) and vesicular stomatitis virus (VSV). Three specific primer sets were selected according to FMDV, SVDV, and VSV gene sequences reported. The results showed that all the samples which contained FMDV, SVDV and VSV could be amplified by this method with optimized factor of reaction. Three specific bands of FMDV 208 bp, and SVDV 862 bp, and VSV 638 bp, as low as 10fg of FMDV-RNA, SVDV-RNA, and VSV-RNA could be detected and visualized under UV light, but not the gene fragments of PRRSV,TGEV and CSFV. The multiplex PCR appears to be rapid, sensitive and specific method for detecting FMDV, SVDV and VSV simultaneously, and may be applied to clinical diagnosis and epidemiological investigation.
>防检技术
>疫病防治
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2006, 23(3):40-40.
摘要:
>口岸之窗
>疫情报道
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2006, 23(3):43-44.
摘要:
>专论综述
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2006, 23(3):45-48.
摘要:
>畜牧资讯
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