2006, 23(4):13-13.
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2006, 23(4):14-15.
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2006, 23(4):15-17.
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薛素强 , 范伟兴 , 洪洁心 , 黄保续 , 蔡振鸿 , 郭霄峰
2006, 23(4):22-24.
摘要:Three pairs of primers were designed and synthesized according to the published literatures and a triple PCR was developed to amplify CPS2J, epf and mrp gene sequences of Streptococus suis Type 2, with the target sequeuces of 230bp, 570bp and 860bp.The triple PCR was tested for its specificity,sensitivity and repeatability.The result showed it could detect as low as 50 germs and with good specificity and repeatability and it could be finished within 7 hours.
陈明 , 黄维义 , 张为宇 , 石云良 , 苏乾莲 , 何木荣
2006, 23(4):25-26.
摘要:In order to study the relationship between E.wenyoni and T.evansi infection in bovine a double PCR assay was established for detecting E.wenyoni and T.evansi infection. 415bp and 237 bp segment was amplified ,and their lowest DNA quantity that could be detected was 0.154pg and 0.105pg. The specific test showed that there were no cross reactions with Mycoplasma hyopneumoniae,E.coli,Staphylococcus, Eimeria gallus, Babesia bigemina et al. Thirty-five clinical samples were detected. The results showed that the positive rate of the E.wenyoni and T.evansi was 22.89% and 8.89% respectively, and 2.89% infected the both diseases. The clinic sample detections have proved that the assay could be used in diagnoses of E.wenyoni and T.evansi infection, especially in early diagnosis.
2006, 23(4):27-28.
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2006, 23(4):31-31.
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2006, 23(4):39-39.
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2006, 23(4):41-43.
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2006, 23(4):47-47.
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