2006年第23卷第7期文章目次

摘要:pET32a recombinant expression vector was constructed and transformed into E.coli BL21 to induce the expression of recombinant infectious bovine rhinotracheitis virus(IBRV) gD protein.According to the sequence of IBRV gD gene reported ,a pair of PCR primers were designed .Then PCR was carried out to amplify an objective fragment of 766 bp.The PCR products were cloned into expression vector pET32a and the recombinant plasmids pet32-gD were abtained.Then, pET 32-gD was transformed into the competent E.coli BL21. Recombinant gD protein induced by IPTG was expressed ,purified and analyzed by western bloting. The results demonstrated that the recombinant protein could react with the antibody against IBRV by western blot,with the good antigenicity.

气相色谱-质谱法测定猪肝中氯丙嗪残留的研究

路平 , 曲志娜 , 谭维泉 , 郑增忍 , 蒋正军

2006, 23(7):30-31.

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禽流感病毒重组核蛋白抗原的纯化及在琼脂免疫扩散试验中的应用

乔彩霞 , 张鹤晓 , 高志强 , 谷强 , 吴丹 , 蒲静 , 郭晋优 , 刘继红