2007年第24卷第7期文章目次

摘要:Based on the sequence data of 18S rRNA gene of Theileria sp,a pair of T.sp-specific primers were designed.These primers selectively amplified a 398bpDNA fragment of the 18S rRNA gene of Theileria sp.No PCR products were amplified from purified DNA of Babesia sp,Anaplasma ovis,Theileria annulata and Trypanosoma.It was estimated that as few as 0.12fg Theileria SP genomic DNA was detectable by PCR.Using this method ,124 blood samples from different area were successfully detected and 24 samples were positive with Theileria sp infection,the others were negative.The results showed that the PCR method developed was an effective tool for diagnosing Theileria SP infection in sheep and goats with high sensitivity and specificity.It will be useful for detecting the acute Theileria SP and latent infected carrier animals.

抗PRRSVGP5和M蛋白独特型抗体替代抗原诱导机体产生中和抗体的研究

周作红 , 管远红 , 龚振华 , 李葳 , 刘俊辉 , 王玉东 , 郭福生 , 郑增忍 , 蒋正军