2007, 24(8):6-7.
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2007, 24(8):18-19.
摘要:
王志亮 , 包静月 , 吴晓东 , 刘雨田 , 李林 , 刘佩兰 , 赵永刚 , 刘春菊 , 肖肖
2007, 24(8):24-26.
摘要:The first outbreak of peste des petits ruminants in ovine in Tibet was confirmed by multiple laboratory tests including one step real-time RT-PCR, conventional RT-PCR, competitive ELISA and virus isolation. The presence of PPRV was detected in 11 out of 13 field samples by one step real-time RT-PCR, among which 8 were further detected by conventional RT-PCR. Sequence analysis showed that the virus fell into the lineage 4 which was epidemic in Middle East and certain western Asian countries. One strain of PPRV was isolated in Vero cell lines from field samples and confirmed by RT-PCR test.
2007, 24(8):27-29.
摘要:In this study, the Arg-deiminase from 9 CHONGQING Streptococcus suis serotype 2 isolates was amplified by PCR. Nucleotide sequencing and analysis results revealed that the Arg-deiminase gene is 1,231bp. The similarity of Arg-deiminase gene and amino acid from 9 CHONGQING isolates was more than 99% and 96% respectively. The Arg-deiminase gene was detected by PCR with a 237bp expected fragment, The results showed that in 35 pathogenic Streptococcus suis isolates from diseased pigs' viscera, 30 isolates were positive; 11 isolates were positive in 14 detected Streptococcus suis isolates from pigs' tonsil. SS1, SS7, SS9, SS13 and SS1/2 detected by PCR were all positive.
2007, 24(8):32-33.
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2007, 24(8):35-36.
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2007, 24(8):38-38.
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2007, 24(8):44-45.
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2007, 24(8):51-51.
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