- 2007年第24卷第9期文章目次
>政策法规
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2007, 24(9):3-3.
摘要:
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2007, 24(9):4-4.
摘要:
>防疫管理
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2007, 24(9):11-11.
摘要:
>行业报道
>肉品安全
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2007, 24(9):15-16.
摘要:
>试验研究
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2007, 24(9):17-19.
摘要:In order to study the situation of rabies virus carried by domestic dogs with healthy appearance in Xiangxi, 38 brain tissue samples collected from places of epidemic focus in September, 2005 and 168 brain tissue samples from markets in January, 2006 were detected with RT-PCR and direct fluorescent antibody test (FAT). Among them five samples were positive. Then five wild rabies virus strains were isolated by mice inoculation test (MIT). Among those five positive samples, four samples, accounting for 10.5% (4 of 38), were from the places of epidemic focuses, and one sample accounting for 0.59% (1 of 168) were from markets. The rate of occurrence of rabies virus in epidemic focuses was in accord with that of other domestic reports, but it was much lower in samples from markets.
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刘旭辉 , 马贵平 , 常津 , 张兵波 , 李冰玲 , 史喜菊 , 刘全国 , 李炎鑫
2007, 24(9):20-22.
摘要:A test method using quantum dots conjugation was developed for the detection of H5N1 subtype avian influenza virus(AIV).CdSe quantum dots(QDs) was used to label chicken anti-AIV IgG, and the production of the CdSe quantum Dots labeled chicken anti-AIV IgG was purified. The purified conjugate of chicken anti-AIV IgG labeled with CdSe quantum dots used as second antibody, and anti-H5N1 subtype AIV mAbs was coated on the ELISA plate. Trachea and lung were the most suitable tissues for the diagnosis of AIV for the quantum dots test. The entie test takes only 3 hours. The sensitivity of this method used to detect AIV positive sample is 4 times higher than that by hemagglutination test. No cross reaction was observed with Newcastle disease virus(NDV),duck plague virus(DPV) and duck hepatitis(DVH).65 clinical samples were detected by this method, and 5 samples were positive, and 60 samples were negative. Compared with virus isolation, this diagnostic accordance rate is 98.46 %. The QDs test method established in this study had been demonstrated to be a specific, sensitive and rapid test for the diagnosis of AIV, and it offers a promising possibility to be used in entry-exit inspection and quarantine.
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张春玲 , 张婉华 , 臧克伟 , 邹勇 , 李春华 , 蒋凤英 , 何锡忠 , 王英
2007, 24(9):23-25.
摘要:In this study , the open reading frame of PPV VP2 and NS1 were amplified by PCR method from the PPV infected ST cells. Then the products were cloned in prokaryotic vector and pET32c/VP2 and pET30a/NS1expressed were construed respectively .The plasmids were introduced into E. coli BL21. After induction by IPTG , the high expression were found in products of pET32c/VP2 and pET30a/NS1 . Biological activity of the recombinant protein were detected by Western-blot , which can be applied in differential diagnosis of PPV infections.
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2007, 24(9):26-28.
摘要:
>防检技术
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2007, 24(9):33-33.
摘要:
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2007, 24(9):34-35.
摘要:
>疫病防治
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2007, 24(9):38-39.
摘要:
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2007, 24(9):40-41.
摘要:
>口岸之窗
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2007, 24(9):42-44.
摘要:
>专论综述
>疫情报道
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2007, 24(9):49-50.
摘要:
>高致病性猪蓝耳病专栏
>OIE宣传
>百家争鸣
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