2008, 25(2):1-2.
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2008, 25(2):4-5.
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2008, 25(2):11-12.
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2008, 25(2):13-16.
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2008, 25(2):21-23.
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王燕 , 张守峰 , 崔艳 , 徐振波 , 于恒智 , 扈荣良 , 张乐萃
2008, 25(2):24-26.
摘要:An recombinant genome of canine adenovirus type-2 was constructed in this experiment. The external part of glycoprotein was amplified through RT-PCR and cloned into Ase I site of pEGFP-C 1-dAse I containing the capsid protein IX of canine adenovirus type-2, pEGFP-SIX was obtained. The capsid protein IX of CAV-2 genome was replaced with the recombinant protein IX that was released by Ase I from pEGFP-SIX rightward between the last codon of the IX gene and the stop codon. As a result, the recombinant genome of CAV-2 was obtained. The results showed that the target genes were cloned into the IX protein by restriction enzyme digestion.
2008, 25(2):38-39.
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2008, 25(2):42-42.
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2008, 25(2):50-50.
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