2010, 27(1):4-5.
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2010, 27(1):6-7.
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2010, 27(1):18-19.
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2010, 27(1):22-24.
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2010, 27(1):24-25.
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2010, 27(1):27-28.
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2010, 27(1):29-30.
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2010, 27(1):31-32.
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2010, 27(1):33-34.
摘要:Diarrhea in calves is commonly caused by enterotoxigenic Escherichia coli (ETEC). A multiplex PCR method was developed to identify enterotoxigenic Escherichia coli strains by amplifying genes encoding F4l and/or K99 fimbriae,heat-stable enterotoxin a (STa),heat-labile enterotoxin (LT). The parameters of PCR amplification, including concentration of dNTP,primers and annealing temperature were optimized.The specificity and sensitivity of the multiplex PCR were determined on the basis of optimized parameters.I...
2010, 27(1):35-38.
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2010, 27(1):45-46.
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2010, 27(1):49-50.
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2010, 27(1):54-54.
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2010, 27(1):61-61.
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2010, 27(1):63-64.
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2010, 27(1):65-67.
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2010, 27(1):73-73.
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