- 2011年第28卷第6期文章目次
>封面与目次
>兽医经济
>他山之石
>兽医卫生监督
>动物福利
>检疫监管
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2011, 28(6):25-26.
摘要:
>体系建设
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2011, 28(6):33-34.
摘要:
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2011, 28(6):35-36.
摘要:
>实验室管理
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2011, 28(6):37-39.
摘要:
>试验研究
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王磊 , 冯娜 , 李天松 , 刘玉秀 , 高玉伟 , 王铁成 , 杨松涛 , 夏咸柱
2011, 28(6):40-43,47.
摘要:Objective To develop new vaccines and improve the current vaccines of canine distemper virus(CDV).Methods With the template of CDV/R-20/8 attenuated strain of canine distemper virus,the full length CDV-F and CDV-H gene fragments were got after the plasmids of pBluescript-F and pBluescript-H were digested by Pme I alone,then the full-length CDV-F and CDV-H gene fragments were directly cloned into pCI-c1-EGFP plasmid respectively and plasmids of pCI-c1-F and pCI-c1-H were constructed,which were identified cor...
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2011, 28(6):44-47.
摘要:Objective To clone and express the brucella outer membrane protein OMP25 and then detect its reactivity to the Brucella immunized rabbit serum.Methods The B.abortus A544 gene was PCR amplified and sequenced,and the prokaryotic expression plasmid pGEX-OMP25 successfully constructed.The plasmid was transferred to the E.coli BL21,and induced with IPTG.High purity protein was obtained by power elution purification.When the purified protein emulsified with Freund's adjuvant was used to immunize rabbits,higher an...
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张永强 , 张维 , 王清华 , 吴研功 , 赵云玲 , 赵永刚 , 王志亮
2011, 28(6):48-49,55.
摘要:Five pairs of primers were designed to diagnose a novel infectious respiratory disease in cattle in a market in Shandong Province.Including general primer for most species of Mycobacterium genus,using the other several pairs of primers,Ureaplasma diversum,M.bovis,M.bovigenitalium,M.bovoculi and CBPP gene segment could be amplified respectively by RT-PCR.All the 64 nasal cotton swab samples were detected by RT-PCR with general primers and 12 were positive.All the 12 samples were tested with the other five pa...
>检验检测
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2011, 28(6):57-58.
摘要:
>疫病防治
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2011, 28(6):62-63.
摘要:
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2011, 28(6):69-70.
摘要:
>专论综述
>疫情报道
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2011, 28(6):90-90.
摘要:
>百家争鸣
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