引用排行
2007, 24(8):24-26.
摘要:
The first outbreak of peste des petits ruminants in ovine in Tibet was confirmed by multiple laboratory tests including one step real-time RT-PCR, conventional RT-PCR, competitive ELISA and virus isolation. The presence of PPRV was detected in 11 out of 13 field samples by one step real-time RT-PCR, among which 8 were further detected by conventional RT-PCR. Sequence analysis showed that the virus fell into the lineage 4 which was epidemic in Middle East and certain western Asian countries. One strain of PPRV was isolated in Vero cell lines from field samples and confirmed by RT-PCR test.
The first outbreak of peste des petits ruminants in ovine in Tibet was confirmed by multiple laboratory tests including one step real-time RT-PCR, conventional RT-PCR, competitive ELISA and virus isolation. The presence of PPRV was detected in 11 out of 13 field samples by one step real-time RT-PCR, among which 8 were further detected by conventional RT-PCR. Sequence analysis showed that the virus fell into the lineage 4 which was epidemic in Middle East and certain western Asian countries. One strain of PPRV was isolated in Vero cell lines from field samples and confirmed by RT-PCR test.
1997(3):6-8.
摘要:
1996(1):27-27.
摘要:
2001, 18(10):24-26.
摘要:
A new viral disease of duck was reported. the strain virus was isolated from infected duck's livers, spleens and pancreas in Fengyang Anhui Province using chicken embryos. The virus could agglutinate the chicken erythrocytes. The hemaagglutination of virus could be inhibited by the convalescent antiserum, the Newcastle disease virus(NDV) F48E9 strain antiserum, the antiserum of goose paramyxovirus type-I isolated by our laboratory respectivcly. But it could' t be inhibited by avian influenza virus(AIV) positive anti- serum. The HA titer ranged between 2~8 and 2~9. Artificial infection could cause up to 100% morbidity and 50% - 100% mortality in 10-day--old ducks, with almost the same clinical symptoms and pathological changes. The 30 - day - old chickens can also be infected with 100% morbidity and 100% mortality. The inactivated vaccine made from the isolated virus and the egg yolk antibodies from its hyperimmunized layers could give very good protection against the natural infection of ducks. The biological characteristics of the virus was determined by measuring the mean death time of chicken embryos(MDT), the intracerebral pathogenicity index in day - old chicks (ICPI) and the intravenous pathogenicity index in 6-week - old chickens(IVPI). The MDT.ICPI and IVPI were 48.9h. 1.8O and 2.45 respectively. The results indicated that the isolate was of high virulence like velogenic Newcastle disease virus(NDV) and named duck paramyxovirus WF00D strain.
A new viral disease of duck was reported. the strain virus was isolated from infected duck's livers, spleens and pancreas in Fengyang Anhui Province using chicken embryos. The virus could agglutinate the chicken erythrocytes. The hemaagglutination of virus could be inhibited by the convalescent antiserum, the Newcastle disease virus(NDV) F48E9 strain antiserum, the antiserum of goose paramyxovirus type-I isolated by our laboratory respectivcly. But it could' t be inhibited by avian influenza virus(AIV) positive anti- serum. The HA titer ranged between 2~8 and 2~9. Artificial infection could cause up to 100% morbidity and 50% - 100% mortality in 10-day--old ducks, with almost the same clinical symptoms and pathological changes. The 30 - day - old chickens can also be infected with 100% morbidity and 100% mortality. The inactivated vaccine made from the isolated virus and the egg yolk antibodies from its hyperimmunized layers could give very good protection against the natural infection of ducks. The biological characteristics of the virus was determined by measuring the mean death time of chicken embryos(MDT), the intracerebral pathogenicity index in day - old chicks (ICPI) and the intravenous pathogenicity index in 6-week - old chickens(IVPI). The MDT.ICPI and IVPI were 48.9h. 1.8O and 2.45 respectively. The results indicated that the isolate was of high virulence like velogenic Newcastle disease virus(NDV) and named duck paramyxovirus WF00D strain.
2007, 24(6):19-21.
摘要:
1998(2):1-3.
摘要:
Lymphocystis disease of Paralichtys olivaceus is an infectious disease of fish caused by lymphocystis virus (LCV) of Iridoviridae.The lymphocystis virus has a wide range of hosts causing serious losses. Outbreaks of the disease occurred in six fish farms. There were distinct cysts on the surface of the infected fish body. The size of the biggest cyst was about 500um in diameter. It was the first report ot the disease in China, and the disease was suspected to be introduced by importation of fishes from other country.The integrated conrtol measures were recommended to control the epidemic and prevent it from spreading and wipe it out as soon as possible.
Lymphocystis disease of Paralichtys olivaceus is an infectious disease of fish caused by lymphocystis virus (LCV) of Iridoviridae.The lymphocystis virus has a wide range of hosts causing serious losses. Outbreaks of the disease occurred in six fish farms. There were distinct cysts on the surface of the infected fish body. The size of the biggest cyst was about 500um in diameter. It was the first report ot the disease in China, and the disease was suspected to be introduced by importation of fishes from other country.The integrated conrtol measures were recommended to control the epidemic and prevent it from spreading and wipe it out as soon as possible.
2002, 19(8):21-23.
摘要:
2002, 19(9):25-28.
摘要:
Vibrio parahaemolyticus is a pathogenic halophile, present widely in bays, coastal areas, salt lakes and sea products, especially in warm regions, offten causing food poisoning of human. Tests of four species of sea products revealed high incidence of the organism, and detection rate was highest in sea shrimps, upto 90%,70% in sea fishes, 60% in molluscs, and 40% in sea crabs. Animal experiment showed that V. parahaemolyticus isolated was of ratetively high pathogenicity.
Vibrio parahaemolyticus is a pathogenic halophile, present widely in bays, coastal areas, salt lakes and sea products, especially in warm regions, offten causing food poisoning of human. Tests of four species of sea products revealed high incidence of the organism, and detection rate was highest in sea shrimps, upto 90%,70% in sea fishes, 60% in molluscs, and 40% in sea crabs. Animal experiment showed that V. parahaemolyticus isolated was of ratetively high pathogenicity.
1999(5):43-45.
摘要:
2000(11):20-22.
摘要:
2003, 20(11):27-29.
摘要:
To establish a more rapid and simple method of detecting antibody of SFV, a dot immunogold filtration assay (DIGFA) was developed by coating the antigen of CSFV on nitrocellulose (NC) membrane, and labeling staphylococcal protein A (SPA) with colloidal gold.The color of the positive dots were so bright that the results could be easily determined.The whole procedure could be finished in 5 min.The method had a better specificity without cross - reaction with positive sera to PKRSV, PRV, PPV, NDV and AFV.Its coincidence with Dot - EUSA,indirect haemagglutination test(IH) were 98.4% and 98.9% respectively.DIGFA,more simple and rapid,was applicable to small diagnostic laboratories.lt could be applied for more early diagnosis ^ epidemiological investigation and the detection of antibody level in vaccinated herds.
To establish a more rapid and simple method of detecting antibody of SFV, a dot immunogold filtration assay (DIGFA) was developed by coating the antigen of CSFV on nitrocellulose (NC) membrane, and labeling staphylococcal protein A (SPA) with colloidal gold.The color of the positive dots were so bright that the results could be easily determined.The whole procedure could be finished in 5 min.The method had a better specificity without cross - reaction with positive sera to PKRSV, PRV, PPV, NDV and AFV.Its coincidence with Dot - EUSA,indirect haemagglutination test(IH) were 98.4% and 98.9% respectively.DIGFA,more simple and rapid,was applicable to small diagnostic laboratories.lt could be applied for more early diagnosis ^ epidemiological investigation and the detection of antibody level in vaccinated herds.
2007, 24(4):23-25.
摘要:
1998(3):1-3.
摘要:
2003, 20(8):36-37.
摘要:
2001, 18(6):43-44.
摘要:
1982(1):36-39.
摘要:
1997(2):8-10.
摘要:
2005, 22(6):17-19.
摘要:
2006, 23(6):31-33.
摘要:
430 serum samples from cattle and sheep were detected by Bengal test(RBT) with antigens from four manufactures in China, resulting in no identical results. The iELISA and cELISA kits for brucellosis from Canadian Brucellosis Reference Lab were used to evaluate the serological methods of RBT and standard tube agglutination(SAT)commonly used in diagnosing brucellosis in China. The RBT, SAT, iELISA and cELISA in detecting the infected cattle, almost obtained the same results (consistency rate was 85%), but SAT and RBT had higher false positive rate (about 15%) and false negative rate (about 10%). The data suggested that RBT screening test could be used in the field, and the positive results should be further confirmed by cELISA.
430 serum samples from cattle and sheep were detected by Bengal test(RBT) with antigens from four manufactures in China, resulting in no identical results. The iELISA and cELISA kits for brucellosis from Canadian Brucellosis Reference Lab were used to evaluate the serological methods of RBT and standard tube agglutination(SAT)commonly used in diagnosing brucellosis in China. The RBT, SAT, iELISA and cELISA in detecting the infected cattle, almost obtained the same results (consistency rate was 85%), but SAT and RBT had higher false positive rate (about 15%) and false negative rate (about 10%). The data suggested that RBT screening test could be used in the field, and the positive results should be further confirmed by cELISA.
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《中国动物检疫》编辑部 ® 2023 版权所有
地址：山东省青岛市南京路369号《中国动物检疫》编辑部 邮编：266032
电话：0532-85623545 0532-85622559 0532-85642906 传真：0532-85621826 鲁ICP备08016398号-3
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